Methods for assaying compounds or agents for ability to...

G - Physics – 01 – N

Patent

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G01N 33/52 (2006.01) G01N 33/15 (2006.01) G01N 33/88 (2006.01)

Patent

CA 2722420

An exemplary embodiment may be directed to a fluorescence polarization assay that screens compounds or agents for their affinity to hematopoietic prostaglandin D synthase (H-PGDS) based on their ability to displace a fluorophore-containing detection analyte bound to an enzyme comprising the primary amino acid sequence of H-PGDS. Another exemplary embodiment utilizes an enzyme having a maltose binding protein amino-acid sequence fused with an N- terminus of the enzyme.

La présente invention concerne, selon un mode de réalisation donné à titre d'exemple, une mesure de la polarisation de fluorescence permettant le criblage de composés ou d'agents sur la base de leur affinité pour la prostaglandine D synthase hématopoïétique (H-PGDS) en se fondant sur leur capacité à déplacer un analyte de détection contenant un fluorophore et lié à une enzyme comprenant la séquence d'acides aminés primaire de l'H-PGDS. Un autre mode de réalisation donné à titre d'exemple a recours à une enzyme comportant la séquence d'acides aminés d'une protéine liant le maltose (MBp) fusionnée à son extrémité N-terminale.

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