Methods for assaying proteinases and proteinase inhibitors

Details Methods for assaying proteinases and proteinase inhibitors Methods for assaying proteinases and proteinase inhibitors

C - Chemistry, Metallurgy

12

Q

C12Q 1/37 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2100469

2100469 9213099 PCTABS00014 Methods are provided for determining proteinases and proteinase inhibitors based on the ability of phenanthradine coumpounds to intercalate between the bases of DNA with a resulting increase in their fluorescence. The DNA is complexed with a protein to block binding of the phenanthradine. Proteinase activity degrades the complexed protein, exposing DNA to phenanthradine binding accompanied by increased fluorescence.

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