Methods for assessing the invasive potential of a cell...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/00 (2006.01)

Patent

CA 2527947

Embodiments of the invention relate to compositions and methods for evaluating or estimating the invasive potential of cells and thereby differentiating between normal and cancerous cells in accordance with the susceptibility of the cellular chromatin to degradation or other modification by particular enzymes or agents. Chromatin within permeabilized normal and non-invasive cells, including chromatin strands removed therefrom, are more susceptible to degradation by endonucleases or proteinases than is the chromatin from invasive cells.

Dans certains modes de réalisation, l'invention concerne des compositions et des méthodes d'évaluation ou d'estimation du potentiel invasif de cellules et par conséquent de différentiation de cellules normales de cellules cancéreuses conformément à la susceptibilité de la chromatine cellulaire à la dégradation ou à une autre modification par des enzymes ou des agents particuliers. La chromatine présente dans des cellules normales et non invasives, perméabilisées, notamment des brins de chromatine éliminés de ces dernières, est plus susceptible d'être dégradée par des endonucléases ou par des protéinases que ne l'est la chromatine de cellules invasives.

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