Methods for attaching nucleic acid molecules to electrically...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07H 19/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12M 1/34 (2006.01) C12P 19/34 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2456204

The present invention relates to a method to attaching nucleic acid molecules to two different electrical conductors, where a first set of oligonucleotide probes is attached to the first electrical conductor with an attachment chemistry which binds the first set of oligonucleotide probes to the first electrical conductors but not to the second electrical conductors.Then, a second set of oligonucleotide probes is attached to the second electrical conductors.The present invention also provides methods for attaching nucleic acid molecules to electrical conductors using a masking agent and methods for attaching nucleic acid molecules to electrical conductors by electrostatic attraction so that the oligonucleotide probes are chemically bound to the electrical conductors.The present invention also discloses methods and devices for detecting a target nucleic acid molecule in a sample.

L'invention porte sur un procédé de fixation de molécules d'acide nucléique à deux conducteurs électriques différents. Selon le procédé, un premier ensemble de sondes oligonucléotidiques est fixé aux premiers conducteurs électriques au moyen d'un processus chimique de fixation qui lie le premier ensemble de sondes oligonucléotidiques aux premiers conducteurs électriques, non aux seconds conducteurs électriques. Puis, un second ensemble de sondes oligonucléotidiques est fixé aux seconds conducteurs électriques. L'invention concerne également des procédés de fixation de molécules d'acide nucléique à des conducteurs électriques au moyen d'un agent masquant, et des procédés de fixation de molécules d'acide nucléique à des conducteurs électriques par attraction électrostatique de manière que les sondes oligonucléotidiques soient chimiquement liées aux conducteurs électriques. L'invention porte aussi sur des procédés et des dispositifs de détection d'une molécule d'acide nucléique cible dans un échantillon.

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