Methods for binding an exogenous molecule to cellular chromatin

Details Methods for binding an exogenous molecule to cellular chromatin Methods for binding an exogenous molecule to cellular chromatin

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/47 (2006.01) C07K 14/71 (2006.01) C07K 14/72 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) C12N 15/66 (2006.01) C12N 15/67 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2407695

Disclosed herein are methods for binding an exogenous molecule to a binding site within a region of interest in cellular chromatin and methods for identifying a binding site in cellular chromatin for an exogenous molecule. The methods include identification of one or more accessible regions in cellular chromatin, identification of a target site within the accessible region, introduction of the exogenous molecule into the cell, and testing for the binding of the exogenous molecule to the binding site.

L'invention concerne des méthodes de fixation d'une molécule exogène à un site de fixation se trouvant dans une région d'intérêt dans la chromatine cellulaire et des méthodes d'identification d'un site de fixation dans la chromatine cellulaire pour une molécule exogène. Les méthodes consistent en l'identification d'une ou de plusieurs régions accessibles dans la chromatine cellulaire, l'identification d'un site cible à l'intérieur de la région accessible, l'introduction de la molécule exogène dans la cellule et l'essai de fixation de la molécule exogène au site de fixation.

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