Methods for capture and selective release of nucleic acids...

Details Methods for capture and selective release of nucleic acids... Methods for capture and selective release of nucleic acids...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2157968

Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with polyethyleneimine to form a precipitate with the nucleic acids. The nucleic acids are then released from the precipitate by contact with a strong base, and the released nucleic acids are kept in solution with an anionic phosphate ester surfactant. This method for preparing specimen samples is simple and quite rapid.

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