C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2158485
Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with specific weakly basic polymers to form a precipitate with the nucleic acids at acidic pH. After removing non-precipitated materials, the pH is then made basic, thereby releasing the nucleic acids from the polymer. This method for preparing specimen samples is simple and quite rapid, and the released nucleic acids can be further treated in hybridization assays or amplification procedures. The weakly basic polymers are water-soluble and cationic at acidic pH, but neutral in charge at basic pH.
Backus John Wesley
Ekeze Tobias E.
Findlay John Bruce
Kerschner Joanne Hansen
Ponticello Ignazio Salvatore
Johnson & Johnson Clinical Diagnostics Inc.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Ortho-Clinical Diagnostics Inc.
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