Methods for characterizing biological molecule modulators

G - Physics – 01 – N

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G01N 33/53 (2006.01) C12Q 1/00 (2006.01) C40B 30/08 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2618315

Methods for characterizing a biochemical reaction and analysis of reaction products by establishing continuously variable concentration gradients of one or more reagents of the biochemical reaction are provided. Methods for determining mechanism of inhibition or activation, potency of inhibition or activation, or both of an enzyme inhibitor or activator, respectively, are also provided. The continuously variable concentration gradients can be established in a microfluidic chip.

Cette invention concerne des méthodes de caractérisation d'une réaction biochimique et d'analyse de produits de réaction, consistant à établir des gradients de concentration à variation continue d'un ou de plusieurs réactifs de la réaction biochimique. Sont également décrites des méthodes de détermination du mécanismes d'inhibition ou d'activation, voire des deux, d'un inhibiteur ou d'un activateur d'enzymes, respectivement. Les gradients de concentration à variation continue peuvent être établis dans une puce microfluidique.

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