Methods for cloning and manipulating genomes

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/10 (2006.01)

Patent

CA 2754212

Compositions and methods are disclosed herein for cloning a donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.

La présente invention concerne des compositions et des procédés permettant le clonage d'un génome donneur dans une cellule hôte hétérologue. Dans un mode de réalisation, le génome donneur peut en outre être modifié dans une cellule hôte. Des génomes modifiés ou non modifiés peuvent en outre être isolés d'une cellule hôte et transférés à une cellule receveuse. Les procédés selon la présente invention peuvent être utilisés pour modifier des génomes donneurs de cellules donneuses difficilement analysables dans des cellules hôtes dont l'analyse est plus aisée.

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