Methods for co-culturing cord blood derived cells with...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/078 (2010.01) C12N 5/071 (2010.01)

Patent

CA 2714777

Methods are provided for obtaining expanded human cord blood cells expressing CD34. The methods involve seeding a sufficient amount of cord blood cells with a sufficient amount of menstrual cells under co-culture conditions suitable to promote expansion of the cord blood cells, and co-culturing the cord blood cells with the menstrual cells under culture conditions that support at least two or more population doublings of the cord blood cells. Methods are also provided for growing expanded human cord blood cells to give rise to any one of colony forming units, colony forming unit granulocyte macrophages (CFU-GM), burst forming unit erythroids (BFU-E), and colony forming unit granulocyte erythrocyte macrophage megakaryocyte (CFU- GEMM) blood lineage precursor cells. The expanded cells may express CD34, SSEA-4, and HLA-II. Compositions of the expanded cells are also provided.

L'invention porte sur des procédés pour obtenir des cellules de sang ombilical humaines expansées exprimant CD34. Les procédés mettent en jeu l'ensemencement d'une quantité suffisante de cellules de sang ombilical avec une quantité suffisante de cellules menstruelles dans des conditions de co-culture appropriées pour favoriser l'expansion des cellules de sang ombilical, et la co-culture des cellules de sang ombilical avec les cellules menstruelles dans des conditions de culture qui supportent au moins deux doublages ou plus de population des cellules de sang ombilical. L'invention porte également sur des procédés pour faire croître des cellules de sang ombilical humaines expansées pour donner naissance à l'une quelconque d'unités de formation de colonies, d'unités de formation de colonies de granulocytes et macrophages (CFU-GM), d'unités précurseurs d'érythroblastes (BFU-E) et de cellules précurseurs de lignée du sang, d'unités de formation de colonies de granulocytes-érythrocytes et macrophages-mégacaryocytes (CFU-GEMM). Les cellules expansées peuvent exprimer CD34, SSEA-4 et HLA-II. L'invention porte également sur des compositions des cellules expansées.

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