C - Chemistry – Metallurgy – 40 – B
Patent
C - Chemistry, Metallurgy
40
B
C40B 40/06 (2006.01) C12M 1/34 (2006.01) C12N 15/00 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) C40B 50/06 (2006.01) C40B 50/14 (2006.01)
Patent
CA 2372593
A method for constructing a cDNA library which comprises hybridizing mRNA with oligo(dT)n on a support and treating with a reverse transcriptase so as to immobilize complementary DNA; or a method for constructing a gDNA library which comprises ligating a double-stranded chromosomal DNA library with an oligonucleotide on a support having a restriction enzyme site and then immobilizing the gDNA library; a method for preparing replicas thereof; and a support carrying the thus replicated DNA fragment thereon.
L'invention concerne un procédé de construction d'une banque d'ADNc, qui consiste à hybrider de l'ARNm avec un oligo(dT)n sur un support et à effectuer un traitement à l'aide de transcriptase inverse, de sorte que l'ADN complémentaire soit immobilisé. L'invention concerne également un procédé de construction d'une banque d'ADNg, qui consiste en la ligature d'une banque d'ADN chromosomique double brin avec un oligonucléotide sur un support possédant un site d'enzyme de restriction, et en l'immobilisation de la banque d'ADNg. L'invention se rapporte encore à un procédé de préparation de répliques à partir de ladite banque et à un support portant ledit fragment d'ADN ainsi répliqué.
Takahashi Kojiro
Takai Osamu
Tanga Michifumi
Nihon Parkerizing Hiroshima Co. Ltd.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
Toyo Kohan Co. Ltd.
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