Methods for controlling sialic acid derivatives in...

C - Chemistry – Metallurgy – 12 – P

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C12P 21/02 (2006.01) C07K 14/505 (2006.01)

Patent

CA 2183552

The invention relates to the effects of CO2 on the carbohydrate composition of a cell culture-derived recombinant glycoprotein. The methods of the invention demonstrate a practical method for controlling levels of a sialic acid derivative, NGNA, in secreted recombinant glycoproteins which will be useful in a variety of bioprocessing systems.

L'invention concerne les effets du CO¿2? sur la composition d'hydrate de carbone d'une glycoprotéine de recombinaison dérivée d'une culture de cellules. Les procédés de l'invention permettent de contrôler et réguler les niveaux d'un dérivé de l'acide sialique, NGNA, dans des glycoprotéines secrétées de recombinaison qui seront utiles dans une varitété de systèmes de biotraitement.

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