Methods for counting cells

G - Physics – 01 – N

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G01N 15/00 (2006.01) G01N 33/48 (2006.01) C12Q 1/06 (2006.01)

Patent

CA 2684465

The invention features methods of quantifying cells in a sample by lysing the cells followed by the measurement of at least one intracellular component. Methods of the invention are especially useful for quantifying small numbers of cells, e.g., over a large surface area or volume compared to the cell size. In a preferred embodiment, methods of the invention are performed using a microfluidic device.

L'invention concerne des procédés de quantification de cellules dans un échantillon par une lyse des cellules suivi de la mesure d'au moins un composant intracellulaire. Les procédés de l'invention sont particulièrement utiles pour la quantification de petits nombres de cellules, par exemple sur une surface spécifique ou un volume important en comparaison avec la taille de la cellule. Dans un mode de réalisation privilégié, les procédés de l'invention sont exécutés à l'aide d'un dispositif microfluidique.

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