Methods for creating recombination products between...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2474898

The invention is directed to the creation of a collection of recombination products between two or more nucleotide sequences. The nucleotide sequences can encode distinct amino acid sequences and the collection of recombination products can be expressed to obtain a corresponding collection of polypeptide recombination products or variants. The amino acid sequences encoded by the two or more nucleotide sequences can correspond to polypeptides that are similar in function, but are encoded by dissimilar nucleotide sequences that cannot be recombined using traditional methods of recombination, which require a high degree of sequence similarity.

L'invention a trait à la création d'une collection de produits de recombinaison entre deux séquences de base ou plus. Les séquences de base peuvent coder des séquences nucléotidiques distinctes, et la collection de produits de recombinaison peut être exprimée pour produire une collection correspondante de produits de recombinaison de polypeptides ou de variantes. Les séquences nucléotidiques codées par les deux séquences de base ou plus peuvent correspondre à des polypeptides dont la fonction est similaire, mais qui sont codés par des séquences de base différentes qui ne peuvent pas être recombinées à l'aide des procédés classiques de recombinaison, qui nécessitent un haut degré de similarité de séquences.

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