C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/10 (2006.01) A01K 67/027 (2006.01) C12N 15/85 (2006.01) C12N 15/867 (2006.01)
Patent
CA 2254866
The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.
La présente invention concerne des procédés et des compositions améliorés servant à la production d'animaux transgéniques non humains. L'invention permet d'introduire des séquences d'acides nucléiques exogènes à l'intérieur du génome d'oeufs non fertilisés (p. ex. des ovocytes en phase de prématuration et des ovocytes en phase de préfertilisation) par micro-injection de rétrovirus infectieux dans l'espace périvitellin de l'oeuf. Les procédés de la présente invention confèrent une efficacité accrue à la production d'animaux transgéniques, et assurent une proportion réduite d'animaux constituant des mosaïques en raison de la présence du transgène.
Bremel Robert D.
Burns Jane C.
Chan Anthony W.s.
Smart & Biggar
The Regents Of The University Of California
Wisconsin Alumni Research Foundation
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