C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 7/01 (2006.01) A61K 39/145 (2006.01) C12N 7/00 (2006.01) C12N 7/02 (2006.01)
Patent
CA 2738022
A novel serum-free cell culture medium and methods for cultivating MDCK cells are presented. In particular, non-tumorigenic MDCK cells. Methods for producing influenza viruses (e.g., particularly cold- adapted, and/or temperature sensitive, and/or attenuated influenza viruses) that eliminate the need for a cell culture medium exchange step are provided. The novel medium and methods are useful to grow influenza viruses, in cell culture to high titer. The present invention further provides purification methods for purifying influenza viruses with high overall recovery of live virus and result in levels of host cell DNA (HCD), host cell protein (HCP) and non-specific endonuclease (e.g., Benzonase), which are below the specifications required by regulatory agencies.
La présente invention concerne un nouveau milieu de culture cellulaire exempt de sérum et des procédés de culture de cellules MDCK, en particulier des cellules MDCK non tumogéniques. La présente invention concerne aussi des procédés de production de certains virus de la grippe, tels que les virus de la grippe particulièrement adaptés au froid, et/ou sensibles à la chaleur, et/ou atténués, qui évitent d'avoir à changer de milieu de culture cellulaire. Le milieu et les procédés de l'invention conviennent particulièrement à la culture des virus de la grippe, avec un titre élevé de culture cellulaire. L'invention concerne également des procédés de purification permettant de purifier les virus de la grippe avec un niveau globalement élevé de récupération du virus vivant, donnant des niveaux d'ADN de cellule hôte ou "HDC" (Host Cell DNA), de protéine de cellule hôte ou "HCP" (Host Cell Protein) et d'endonucléase non spécifique telle que la Benzonase inférieurs aux spécifications imposées par les organismes de régulation.
Cataniag Floro
Hsu Simon S.
Liu Jonathan
Maranga Luis J.
Thompson Mark
Medimmune Llc
Smart & Biggar
LandOfFree
Methods for cultivating cells, propagating and purifying... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for cultivating cells, propagating and purifying..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for cultivating cells, propagating and purifying... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1652086