Methods for culturing cells

Details Methods for culturing cells Methods for culturing cells

C - Chemistry, Metallurgy

07

K

C07K 14/81 (2006.01) A61K 38/57 (2006.01) A61K 35/12 (2006.01) A61K 38/00 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2267332

The invention relates to methods for culturing cells, in particular to the expansion of hematopoietic cells, specifically stem cells and/or progenitor cells. These cells can be inhibited in their outgrowth by the presence in the medium of proteases. Methods to avoid this inhibition and related uses of this finding are disclosed.

L'invention a trait à des procédés destinés à la culture de cellules, en particulier à l'expansion de cellules hématopoïétiques, spécifiquement des cellules souches et/ ou des cellules précurseur. Ces cellules peuvent être inhibées dans leur croissance par la présence de protéases dans le milieu de culture. Des procédés destinés à éviter une telle inhibition, ainsi que des utilisations liées à cette découverte sont décrits.

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