C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/00 (2006.01) G01N 33/53 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)
Patent
CA 2419062
Methods for culturing human lung mast cells in the absence of co-culture with feeder cells which are viable for longer than 4 days are provided. Also provided are methods for using these human lung mast cell cultures to assess biological and pharmacological activities of these cells in vivo and to identify modulators of the survival, proliferation, function and phenotypic expression of human lung mast cells. Modulators identified via these cells are useful in the prevention and treatment of diseases involving human lung mast cell function.
L'invention porte sur des procédés de mise en culture de mastocytes de poumons humains, en absence de co-culture de cellules nourricières, viables pendant plus de 4 jours. L'invention porte également sur des procédés d'utilisation desdites cultures pour vérifier les activités biologiques et pharmaceutiques desdits mastocytes in vivo, et en identifier les modulateurs de survie, de prolifération, des fonctions, et d'expression des phénotypes. Les modulateurs ainsi identifiés s'avèrent utiles pour la prévention et le traitement de maladies liées aux fonctions des mastocytes de poumons humains.
Borden Ladner Gervais Llp
Philadelphia Health And Education Corporation
LandOfFree
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