Methods for delivering nucleic acid molecules into cells and...

A - Human Necessities – 61 – K

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A61K 48/00 (2006.01) A61K 41/00 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12N 15/63 (2006.01) C12N 15/85 (2006.01) C12N 15/87 (2006.01) C12N 15/88 (2006.01)

Patent

CA 2441535

Methods for delivering nucleic acid molecules into cells and methods for measuring nucleic acid delivery into cells and the expression of the nucleic acids are provided. The methods are designed for introduction of large nucleic acid molecules, including artificial chromosomes, into cells, and are practiced in vitro and in vivo.

La présente invention concerne des techniques d'apport de molécules d'acides nucléiques dans des cellules et des techniques permettant de mesurer l'apport d'acides nucléiques dans ces cellules et l'expression de ces acides nucléiques. Ces techniques sont conçue en vue d'introduire dans des cellules de grosses molécules d'acides nucléiques, y compris des chromosomes artificiels. Ces techniques sont mises en oeuvre <i>in vitro</i> et <i>in vivo</i>.

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