Methods for detecting abnormally methylated nucleic acid in...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C40B 30/00 (2006.01)

Patent

CA 2482192

The invention provides amplification-based methods for detecting hypermethylated or hypomethylated nucleic acid in heterogeneous biological samples, e.g., stool. A screening procedure based on the detection of hypermethylation, preferably at multiples genes, provides a means for detecting various diseases, e.g., colorectal cancer. By using chimeric primers that contain a 5' non-specific portion, the specificity and efficiency of the nucleic acid amplification is improved. Methods of the invention are especially useful in detection of rare events in a heterogeneous sample.

L'invention concerne des procédés basés sur l'amplification destinés à détecter des acides nucléiques hyperméthylés ou hypométhylés dans des échantillons biologiques hétérogènes, par exemple des fèces. Une procédure de criblage basée sur la détection d'hyperméthylation, de préférence sur de multiples gènes, constitue un moyen de détecter différentes maladies, par exemple, le cancer colorectal. L'utilisation d'amorces chimères contenant une portion non spécifique 5' permet d'améliorer la spécificité et l'efficacité de l'amplification d'acides nucléiques. Les procédés de l'invention sont particulièrement utiles dans la détection d'évènements rares dans un échantillon hétérogène.

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