G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/58 (2006.01) G01N 15/10 (2006.01) G01N 33/53 (2006.01) G01N 33/535 (2006.01) G01N 33/569 (2006.01)
Patent
CA 2358510
The present invention relates to methods of tyramide coating live cells for flow cytometry, using catalyzed reporter deposition and serial amplification staining. A catalyzed reporter deposition or an analyte dependent enzyme activation system is described for detecting and/or quantitating an analyte of interest on the surface of a cell by flow cytometry. Also described is a method for serial amplification staining by tyramide coating cells which possess an analyte of interest or a solid phase to which an analyte is bound.
La présente invention concerne des méthodes de déposition de couche de tyramide sur des cellules vivantes destinées à la cytométrie de flux, au moyen de dépôt de rapporteur et de coloration par amplification en série. Un rapporteur catalysé ou un système d'activation dépendant d'un analyte permet de détecter et/ou de quantifier un analyte recherché sur la surface d'une cellule au moyen de la cytométrie de flux. L'invention concerne aussi une méthode de coloration par amplification en série par revêtement à la tyramide de cellules possédant un analyte recherché ou une phase solide à laquelle un analyte est lié.
Kaplan David R.
Smart & Biggar
Verve Ltd.
LandOfFree
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