Methods for detecting and measuring spliced nucleic acids

Details Methods for detecting and measuring spliced nucleic acids Methods for detecting and measuring spliced nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2337106

A simplified method for preparing nucleic acid, preferably spliced mRNA, from a biological sample that is suitable for amplification is disclosed. A method of detecting and measuring the amount of one or more species of spliced mRNA present in the sample, following nucleic acid amplification, is disclosed.

On décrit un procédé simplifié de préparation d'un acide nucléique, de préférence un ARNm épissé, à partir d'un prélèvement biologique se prêtant à une amplification. On décrit en outre un procédé de détection et de mesure de la quantité d'une ou de plusieurs espèces d'ARNm épissés présents dans le prélèvement, après amplification de l'acide nucléique.

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