Methods for detecting colon cancer from stool samples

G - Physics – 01 – N

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G01N 33/574 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2215263

The present invention provides methods for screening for the presence of a subpopulation of cancerous or precancerous cells in a heterogeneous cellular sample, such as a stool sample. The methods take advantage of the recognition that cellular debris from cancerous and precancerous cells is deposited onto only a longitudinal stripe of stool as the stool is forming in the colon. Accordingly, methods of the invention comprise obtaining a representative sample, such as a cross-sectional sample of stool in order to ensure that any cellular debris that is shed by colonic cells is obtained in the sample.

La présente invention concerne des procédés de tri permettant de détecter la présence d'une sous-population de cellules cancéreuses ou précancéreuses dans un échantillon cellulaire hétérogène, tel qu'un échantillon de selles. Ces procédés tirent avantage du fait que les débris cellulaires de cellules cancéreuses ou précancéreuses ne se déposent que sur une bande longitudinale des selles lors de la formation de ces dernières dans le côlon. Les procédés décrits dans la présente invention consistent à obtenir un échantillon représentatif, tel qu'un échantillon de selles de section transversale, qui permette de s'assurer que tout débris cellulaire caché par les cellules du côlon soit présent dans l'échantillon.

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