Methods for detecting dihydroxyvitamin d metabolites by mass...

G - Physics – 01 – N

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G01N 27/00 (2006.01) G01N 1/34 (2006.01) G01N 33/74 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2706953

Provided are methods of detecting the presence or amount of a dihydroxyvitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydroxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.

L'invention concerne des procédés de détection de la présence ou d'une quantité d'un métabolite de dihydroxyvitamine D dans un échantillon au moyen de la spectrométrie de masse. Les procédés comprennent généralement l'ionisation d'un métabolite de dihydroxyvitamine D dans un échantillon et la détection de la quantité de l'ion afin de déterminer la présence ou la quantité du métabolite de vitamine D dans l'échantillon. Dans certains modes de réalisation préférés, les procédés comprennent l'immunopurification des métabolites de dihydroxyvitamine D avant la spectrométrie de masse. L'invention concerne également des procédés pour détecter la présence ou une quantité de deux métabolites de dihydroxyvitamine D ou plus par une seule analyse.

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