Methods for detecting isolates of the potato virus y (pvy)...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/70 (2006.01) C12N 9/64 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2604027

The invention concerns a method for detecting the PVY virus which consists in a SNP test or an extension reaction specific to mutations corresponding to R/K400 et D/E419, the presence of at least one of said mutations indicating the presence of at least one virulent PVY strain capable of causing necrosis in plants of the Solanaceae family, in particular in potatoes.

La présente invention concerne un procédé de détection du virus PVY consistant en un test SNP ou une réaction d'extension spécifique des mutations correspondant à R/K400 et D/E419, la présence d'au moins une desdites mutations étant une indication de la présence d'une souche de PVY virulente pouvant être responsable de la nécrose chez les plantes de la famille des Solanacées, en particulier chez la pomme de terre.

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