Methods for detecting membrane derived caspase activity and...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/37 (2006.01)

Patent

CA 2366918

Provided are methods for detecting membrane derived apoptotic activity. In one embodiment, the present invention provides methods for identifying membrane derived caspase activity. In other embodiments, drug discovery methods are provided for screening compounds that inhibit or enhance membrane derived caspase activity. In the various embodiments, heavy membrane fractions are utilized for the screening methodologies described herein.

L'invention concerne des procédés permettant de détecter une activité d'apoptose d'origine membranaire. Dans un mode de réalisation, l'invention concerne des procédés pour identifier une activité de caspase due à une membrane. Dans d'autres modes de réalisation, l'invention concerne des procédés de découverte de médicaments permettant de cribler les composés inhibant ou renforçant l'activité de caspase d'origine membranaire. Dans divers modes de réalisation, on utilise des fraction lourdes de membranes dans les méthodes de criblage décrites dans l'invention.

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