Methods for detecting molecular complexes

G - Physics – 01 – N

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Details

G01N 33/564 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01) C40B 30/04 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2655563

The invention includes methods for analyzing copies of an antibody microarray for a protein complexed with another molecule. In this method, one biological sample is divided and then incubated with copies of an antibody microarray. Each capture antibody is paired with a detection antibody, and only one detection antibody is incubated with each copy of the antibody microarray. The invention further includes methods to compare protein complexes under different conditions, methods of detecting an auto-immune response, and methods of detecting interaction between a protein and a small molecule.

La présente invention concerne des procédés permettant d'analyser des copies d'un microréseau d'anticorps dirigés contre une protéine complexée avec un autre molécule. Dans ce procédé, un échantillon biologique est divisé puis incubé avec des copies d'un microréseau d'anticorps. Chaque anticorps capturé est couplé à un anticorps de détection et seul un anticorps de détection est incubé avec chaque copie du microréseau d'anticorps. L'invention concerne en outre des procédés permettant de comparer des complexes protéiques dans différentes conditions, des procédés de détection d'une réponse auto-immune, et des procédés de détection des interactions entre une protéine et une petite molécule.

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