C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 7/06 (2006.01) C07K 16/10 (2006.01) C12N 5/18 (2006.01) G01N 33/569 (2006.01) G01N 33/576 (2006.01) G01N 33/577 (2006.01)
Patent
CA 2491918
A method for treating a hepatitis C or hepatitis B virus-containing sample, characterized by treatment of the virus-containing sample with a treatment solution containing (1) an anionic surfactant and (2) an amphoteric surfactant, nonionic surfactant or protein denaturant, and a virus assay method using said treating method. Also provided is a method for treating a hepatitis C virus-containing sample, characterized by treatment of the virus-containing sample with a treatment solution containing (1) a chaotropic ion and (2) an acidifying agent, and a virus assay method using said treating method. Further provided is a hepatitis C virus assay method comprising treating a hepatitis C virus antigen-containing sample with a surfactant having an alkyl group of or more carbon atoms and a secondary, tertiary or quaternary amine, or a nonionic surfactant, or of both of them, and measuring binding of the antigen with a hepatitis C virus antibody. Finally, there is also provided a monoclonal antibody and a hybridoma producing the same for carrying out the above methods.
Aoyagi Katsumi
Iida Kumiko
Kimura Tatsuji
Ohue Chiharu
Yagi Shintaro
Advanced Life Science Institute Inc.
G. Ronald Bell & Associates
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