Methods for detection of antiestrogen-resistant breast cancer

G - Physics – 01 – N

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G01N 33/574 (2006.01) A61K 38/17 (2006.01) A61K 38/18 (2006.01) A61K 48/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2354084

Disclosed are methods for the detection, diagnosis and prediction of tamoxifen- resistant breast cancer. Genetic and antibody probes and methods useful in determining the presence and monitoring the progression of breast cancer are also described. The methods involve determining polypeptide or mRNA expression of the genes encoding the angiogenic agents or receptors TIE-2, EDNRA, TGF.beta.3, TGFR.beta.III, VEGFR1, VEGF or bFGFR. Also described are procedures for combination therapies utilizing antiangiogenic agents or gene therapy directed towards TIE-2, EDNRA, TGF.beta.3, TGFR.beta.III, VEGFR1, VEGF or bFGFR, in combination with tamoxifen treatment of breast cancer.

L'invention concerne des procédés et des compositions destinés à la détection, au diagnostic et à la prévision du cancer du sein résistant au tamoxifène. Elle concerne aussi des sondes génétiques et à base d'anticorps ainsi que des procédés utiles pour déterminer la présence et la surveillance de la progression du cancer du sein. Les procédés consistent à déterminer l'expression polypeptidique ou par ARNm des gènes codant pour les agents angiogéniques ou les récepteurs TIE-2, EDNRA, TGF.beta.3, TGFR.beta.III, VEGFR1, VEGF or bFGFR. Enfin, elle concerne des procédures pour thérapies combinées utilisant des agents anti-angiogéniques ou la thérapie génique visant TIE-2, EDNRA, TGF.beta.3, TGFR.beta.III, VEGFR1, VEGF ou bFGFR, en combinaison au traitement du cancer du sein par tamoxifène.

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