Methods for detection of breast cancer

G - Physics – 01 – N

Patent

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G01N 33/574 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2501330

The invention relates to a simple screening test for neoplasia, a precancerous condition, or cancer of the breast. A method is described whereby a breast cancer marker is detected in breast fluid. In a particular embodiment, the method involves treating samples of breast fluids with an aldehyde detecting reagent without any prewashing. The appearance in breast fluids of a marker that is detected by an aldehyde detecting reagent, such as a Schiff's reagent, correlates very well with the disease status of the breast cancer subjects from which the fluids were obtained. Screening test kits are also provided.

L'invention concerne un test de criblage simple d'une néoplasie, d'un état précancéreux ou d'un cancer du sein. L'invention concerne notamment une méthode consistant à détecter un marqueur du cancer du sein dans un fluide mammaire. Dans un mode de réalisation, cette méthode consiste à traiter des échantillons de fluides mammaires avec un réactif détectant les aldéhydes, sans étape de prélavage. L'apparition dans des fluides mammaires d'un marqueur détecté par un réactif détectant les aldéhydes, tel qu'un réactif de Schiff, correspond exactement à l'état pathologique des sujets atteints d'un cancer du sein auprès desquels les fluides ont été obtenus. L'invention concerne également des nécessaires de test de criblage.

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