Methods for detection of target on responsive polymeric...

C - Chemistry – Metallurgy – 40 – B

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C40B 40/06 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01) C40B 30/04 (2006.01) C40B 50/14 (2006.01) C40B 60/12 (2006.01) C40B 70/00 (2006.01) G01N 33/52 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2676442

Methods and tools (e.g., kits, articles of manufacturing, support and arrays) for the solid-phase detection of a target molecule using a cationic polymer and nucleic acid probe complex is provided herewith. These methods and tools allows for the reagentless, ultrasensitive and specific detection of nucleic acids, proteins and other molecules of interest and are based on a labeled complex made of specific capture probes and a polythiophene derivative.

L'invention concerne des procédés et des outils (par exemple des kits, des articles manufacturés, un support et des réseaux) destinés à la détection en phase solide d'une molécule cible au moyen d'un complexe formé par un polymère cationique et un acide nucléique sonde. Ces procédés et outils permettent de détecter des acides nucléiques, protéines et autres molécules cibles de façon spécifique, ultra-sensible et sans utiliser de réactifs; ils sont basés sur un complexe marqué formé de sondes de capture spécifiques et d'un dérivé polythiophène.

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