Methods for determining compounds capable of inhibiting the...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/10 (2006.01) C07K 14/47 (2006.01) C07K 14/82 (2006.01) C12N 15/85 (2006.01) C12Q 1/26 (2006.01) C12Q 1/37 (2006.01) C12Q 1/68 (2006.01) G01N 33/573 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2238290

This invention provides a method for determining whether a compound is capable of suppressing ras functions comprising: (a) contacting an effective amount of the compound with Ha-ras transformed cloned rat embryo fibroblast cells under conditions permitting the compound to suppress ras functions in the cells; and (b) determining the expression or inhibition of certain indicator gene or genes, thereby determining whether the compound is capable of suppressing ras function. This invention further provides the determined compound and a pharmaceutical composition comprising the compound and a pharmaceutically acceptable carrier. This invention also provides methods for generating transcriptional switched Ha-ras transformed cloned rat embryo fibroblast cells. This invention also provides the generated cells and different uses of the cells.

L'invention concerne un procédé pour déterminer si un composé est capable de supprimer les fonctions ras, qui consiste à: (a) mettre une dose efficace du composé en contact avec des cellules fibroplasiques embryonnaires de rats clonés, transformées par Ha-ras dans des conditions permettant au composé de supprimer les fonctions ras dans les cellules; et (b) déterminer l'expression ou l'inhibition d'un ou de plusieurs gènes indicateurs, et à déterminer ainsi si le composé est capable de supprimer la fonction ras. L'invention porte également sur le composé déterminé, sur une composition pharmaceutique le comprenant et sur un vecteur acceptable du point de vue pharmaceutique. Elle se rapporte encore à des procédés de génération de cellules fibroplasiques embryonnaires de rats clonés transformées par Ha-ras, aux cellules générées et aux différentes utilisations de celles-ci.

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