Methods for determining oxidative injury to dna

G - Physics – 01 – N

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G01N 33/483 (2006.01) C12Q 1/68 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2186751

The invention relates to a diagnostic and prognostic method for detecting breast cancer at an early stage of its development utilizing analysis of intact DNA specimens by vibrational spectroscopy to determine the lesion profile of the DNA. The method also identifies certain types of DNA lesions, evidenced in spectra profiles having increased infrared absorption, that are characteristic of neoplastic specimens. Tissues having DNA absorption values approaching those values associated with established neoplastic DNA values in these regions are at a heightened risk of impending metastasis or of developing neoplasia unless cellular conditions in the tissue are altered to reduce DNA modifications.

L'invention concerne une technique de diagnostic et de pronostic permettant de détecter un cancer du sein au stade initial de son développement, au moyen d'une analyse de spécimens d'ADN intacts, par spectroscopie vibratoire afin de déterminer le profil de lésion de l'ADN. Cette technique identifie également certains types de lésions de l'ADN, mises en évidence dans des profils de spectre présentant une absorption accrue des infrarouges, caractéristiques de spécimens néoplasiques. Des tissus présentant des valeurs d'absorption de l'ADN proches des valeurs associées à des valeurs d'ADN néoplasique établies dans ces zones, présentent un risque élevé de développer des métastases de façon imminente ou des néoplasies, à moins que les conditions cellulaires dans le tissu ne soient modifiées afin de réduire les modifications de l'ADN.

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