C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/02 (2006.01) G01N 33/04 (2006.01) G01N 33/68 (2006.01) C07K 14/495 (2006.01)
Patent
CA 2741548
A novel method for de-termining the bioactivity of TGF-.beta. in a sample of milk, raw protein source, or nutritional composition is provided. The method includes particular reconstitution steps, centrifugation steps, incubation steps, and activation steps. The bioactiv-ity of the TGF-.beta. in the sample may be measured in a HT-2 cell bioassay or a cellomics bioassay.
Linvention porte sur un procédé permettant de déterminer la bioactivité du TGF-ß dans un échantillon de lait, de source de protéine brute ou de composition nutritionnelle. Le procédé comprend des étapes de reconstitution, des étapes de centrifugation, des étapes dincubation et des étapes dactivation particulières. La bioactivité du TGF-ß dans léchantillon peut être mesurée dans un dosage biologique de cellule HT-2 ou dans un dosage biologique Cellomics.
Jouni Zeina E.
Rai Gyan P.
Rosales Francisco J.
Waworuntu Rosaline
Gowling Lafleur Henderson Llp
Mead Johnson Nutrition Company
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