Methods for determining the biocompability of lipophilic...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/02 (2006.01) C12N 1/38 (2006.01) C12N 5/00 (2006.01) C12P 33/00 (2006.01) C12Q 1/18 (2006.01) G01N 33/50 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2264441

Nanoemulsions are used for testing the cell toxicity of lipophilic substances in cell culture assays; for testing the genotoxicity of lipophilic substances in cell culture as-says; for the biotransformation of lipophilic substances by using cell cultures; promotion of cell differentiation in primary cell cultures; for completion of cell cultures with lipophilic substances promoting the cell growth; for completion of cell cultures with lipophilic substances promoting the biosynthesis of desired substances within the cells; as well as for the defined and reproducible uptake of lipophilic substances into the cells of cell cultures. Preferred nanoemulsions have oily particles of a diameter of less than 100 nm, the surface of which is bearing a monolayer of an amphoteric emulsifier, which oily particles comprise a lipophilic substance to be tested in or to be delivered to a cell culture, said oily particles, said lipophilic substance dissolved therein and the aqueous phase being sterile.

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