Methods for determining the response of cells to vegf and...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) A61P 35/00 (2006.01) C12N 15/11 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2475626

The present invention provides methods of monitoring the progression of a disease condition associated with angiogenesis or vassculogenesis in a human subject in which a quantitative determination of the transcript level of at least one gene shown in Table 1 (by which is meant one or more of any of Tables 1a to 1f) in a sample comprising cells obtained from the site of said disease is made, and compared with the transcript level of at least one gene obtained from a control sample of cells. The transcripts of Table 1 are found to response to VEGF in a statistically significant manner under a variety of different conditions, including following serum withdrawal. The invention also provides gene chip arrays consisting of all or some of the transcripts together with appropriate controls which can be used in the methods described.

La présente invention concerne des procédés qui permettent de surveiller l'évolution d'un état pathologique associé à l'angiogénèse ou à la vasculogénèse chez un sujet humain, selon lesquels on procède à une détermination quantitative du niveau de transcription d'au moins un gène illustré dans le tableau 1 (c'est-à-dire un ou plusieurs gènes d'un quelconque tableau parmi les tableaux 1a à 1f) dans un échantillon contenant des cellules extraites du site de la maladie, et on le compare au niveau de transcription d'au moins un gène obtenu à partir d'un échantillon de cellules témoin. Il a été découvert que les transcrits du tableau 1 répondent au facteur de croissance endothéliale (VEGF) d'une manière statistiquement significative dans une variété de conditions différentes, y compris suite à une déprivation en sérum. L'invention se rapporte également à des biopuces composées d'une partie ou de la totalité des transcrits et des témoins appropriés, qui peuvent être utilisées dans les procédés précités.

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