Methods for developing conifer somatic embryos

A - Human Necessities – 01 – H

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A01H 4/00 (2006.01) A01H 7/00 (2006.01)

Patent

CA 2496880

The present invention provides methods for producing conifer cotyledonary somatic embryos. The methods of the present invention each includes the step of culturing embryogenic conifer tissue in, or on, a development medium for a period of time sufficient to produce conifer cotyledonary somatic embryos from the embryogenic conifer tissue, wherein the volume of development medium is in the range of from about 35 ml to about 50 ml per 0.5 mL of embryogenic conifer tissue.

Méthodes de production d'embryons somatiques cotylédonaires de conifères. Chacune de ces méthodes comprend l'étape de la culture de tissu embryogène de conifères dans ou sur un milieu de développement pendant une période de temps suffisante pour produire des embryons somatiques cotylédonaires de conifères à partir du tissu embryogène. Le volume du milieu de développement est de 35 à 50 ml par 0,5 ml de tissu embryogène de conifères.

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