Methods for direct detection of individual methotrexate...

G - Physics – 01 – N

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Details

G01N 33/48 (2006.01) G01N 21/31 (2006.01) G01N 21/64 (2006.01) G01N 27/00 (2006.01) G01N 33/49 (2006.01) G01N 30/02 (2006.01)

Patent

CA 2517709

The present invention provides a method for determining a level of a methotrexate polyglutamate (MTXPG) in a cellular extract by resolving at least one MTXPG in a cellular extract obtained from a human undergoing methotrexate therapy; and detecting at least one resolved MTXPG, thereby determining a level of the resolved MTXPG. A method of the invention can be useful, for example, for determining a level of MTXPG3. MTXPG4 or MTXPG5 or for determining a level of each methotrexate polyglutamate species (MTXPG1 to MTXPG7) present in the cellular extract.

La présente invention concerne une méthode destinée à déterminer un taux d'un polyglutamate de méthotrexate (MTXPG) dans un extrait cellulaire par résolution d'au moins un MTXPG dans un extrait cellulaire obtenu à partir d'un être humain soumis à un traitement au méthotrexate, et à détecter au moins un MTXPG résolu, ce qui permet de déterminer un taux du MTXPG résolu. Une méthode de l'invention peut être utile, par exemple, pour déterminer un taux de MTXPG¿3?, MTXPG¿4? ou MTXPG¿5?, ou pour déterminer un taux de chaque espèce de polyglutamate de méthotrexate (MTXPG¿1? à MTXPG¿7?) présente dans l'extrait cellulaire.

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