Methods for encapsulating nucleic acids in lipid bilayers

C - Chemistry – Metallurgy – 12 – N

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C12N 15/88 (2006.01) A61K 9/127 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2309727

The present invention relates to lipid-based formulations for nucleic acid delivery to cells, methods for the preparation of such formulations and, in particular, to lipid encapsulated plasmids. The compositions are safe and practical for clinical use. In addition, the present invention provides methods for introducing nucleic acids into cells and for inhibiting tumor growth in cells using such lipid-nucleic acid formulations.

La présente invention concerne des formulations à base de lipides destinées à l'apport d'acides nucléiques dans des cellules, des méthodes de préparation de ces formulations et, en particulier, des plasmides encapsulés dans des lipides. Ces compositions sont sans danger et pratiques dans des applications cliniques. En outre, la présente invention concerne des méthodes d'introduction d'acides nucléiques dans des cellules, ainsi que des méthodes d'inhibition de la croissance tumorale dans des cellules utilisant ces formulations de lipides-acides nucléiques.

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