Methods for enhanced production of bone morphogenetic proteins

Details Methods for enhanced production of bone morphogenetic proteins Methods for enhanced production of bone morphogenetic proteins

C - Chemistry, Metallurgy

12

P

C12P 21/02 (2006.01) A61K 38/18 (2006.01) C07K 14/51 (2006.01) C12N 1/38 (2006.01) C12N 5/00 (2006.01)

Patent

CA 2720980

Methods and processes for improved recombinant protein production are provided. The methods are useful for production of growth factors, particularly those of the TGF-.beta. superfamily, including bone morphogenetic proteins (BMPs), such as BMP-2. Suitable host cells are cultured in media where iron is present at a concentration of at least 2.25 µM and if pyridoxal is present, it makes up less than 55 % of the molar concentration of vitamin B6 in the media.

L'invention concerne des procédés et des traitements pour la production améliorée de protéines recombinantes. Les procédés sutilisent pour la production de facteurs de croissance, en particulier ceux de la super famille TGF-ß, comprenant les protéines morphogénétiques osseuse (BMP), telles que les BMP-2. Des cellules hôtes appropriées sont cultivées dans des milieux où le fer est présent à une concentration d'au moins 2,25 µM et si du pyridoxal est présent, il constitue moins de 55 % de la concentration molaire de vitamines B6 dans le milieu.

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