Methods for enhancing plant transformation frequencies

Details Methods for enhancing plant transformation frequencies Methods for enhancing plant transformation frequencies

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 15/54 (2006.01)

Patent

CA 2414471

The invention provides improved transformation methods. In particular the method provides increased transformation frequency, especially in recalcitrant plants. The method comprises stably transforming a target cell with one or more vectors containing at least one polynucleotide of interest. The target cell has been previously modified to stimulate growth of the cell and has gone through at least one cell division.

L'invention concerne des méthodes de transformation améliorées. Plus particulièrement, elle se rapporte à une méthode permettant d'augmenter la fréquence de transformation, notamment dans les plantes récalcitrantes. Cette méthode consiste à transformer, de manière stable, une cellule cible à l'aide d'un ou de plusieurs vecteurs contenant au moins un polynucléotide d'intérêt. On a préalablement modifié cette cellule cible en vue de stimuler sa croissance, ladite cellule ayant subi au moins une division cellulaire.

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