Methods for enhancing targeted gene alteration using...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/00 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2417344

Methods are presented for enhancing the efficiency of oligonucleotide-mediated repair or alteration of genetic information. The methods comprise using cells or cell-free extracts having altered levels or activity of at least one protein from the RAD52 epistasis group, the mismatch repair group or the nucleotide excision repair group. Kits and compositions are also presented.

La présente invention concerne des méthodes d'augmentation de l'efficacité de la réparation ou de l'altération d'informations génétiques dirigée à l'aide d'oligonucléotides. Ces méthodes consistent à utiliser des extraits cellulaires ou acellulaires présentant une activité ou des niveaux modifiés d'au moins une protéine du groupe d'épistasie RAD52, du groupe de réparation des mésappariements ou du groupe de réparation de l'excision nucléotidique. De plus, l'invention concerne des kits et des compositions associés.

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