Methods for evaluating angiogenic potential in culture

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/02 (2006.01) C40B 30/04 (2006.01)

Patent

CA 2680528

The present invention provides a method of evaluating the angiogenic potential of a tumor, and for predicting the efficacy of anti-angiogenic therapies on an individualized basis. The method of the invention involves preparing an angiogenic signature for malignant cells in culture by assaying for the presence or level of one or more angiogenesis-related factors selected from VEGF/VPF, IL8/CXCL8, TGF-.beta.1, TGF-.beta.2, TGF-.beta.3, bFGF/FGF-2, EGF, PDGF-AA, PDGF-AA/BB, IP-10, and Flt-3 ligand. The angiogenic signature may be prepared from cultures maintained under normoxic and/or hypoxic environments. The invention may be used in conjunction with chemoresponse testing of anti-tumor agents, to predict or suggest a combination therapy for cancer patients.

La présente invention concerne un procédé destiné à évaluer le potentiel angiogénique d'une tumeur, et à prédire l'efficacité de thérapies anti-angiogéniques sur une base individuelle. Le procédé selon l'invention comprend la préparation d'une signature angiogénique pour des cellules malignes en culture en dosant la présence ou le niveau d'un ou plusieurs facteurs associés à l'angiogenèse choisis parmi les ligands des VEGF/VPF, IL8/CXCL8, TGF-.beta.1, TGF-.beta.2, TGF-.beta.3, bFGF/FGF-2, EGF, PDGF-AA, PDGF-AA/BB, IP-10, et Flt-3. La signature angiogénique peut être préparée à partir de cultures maintenues dans des environnements normoxiques ou hypoxiques. L'invention peut être utilisée conjointement à des tests de chimioréponse à des agents anti-tumoraux, afin de prédire ou de suggérer une thérapie mixte pour des patients atteints d'un cancer.

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