Methods for extracting and purifying kallidinogenase

Details Methods for extracting and purifying kallidinogenase Methods for extracting and purifying kallidinogenase

C - Chemistry, Metallurgy

12

P

195/123, 195/75

C12P 21/00 (2006.01) C12N 9/64 (2006.01)

Patent

CA 1057221

Abstract of the Disclosure A method for extracting and collecting kallidinogenase (EC 3.4.4.21 substance) which comprises extracting the pancreas of a mammal with water in the presence of a salt at a temperature of about 45 to about 60°C. and a pH of about 5 to about 8, wherein the extraction is carried out in the presence of a water-soluble salt of magnesium with an acid, and a method for purifying kallidinogenase which comprises subjecting the aqueous phase of a kallidinogenase-containing aqueous extract obtained from the pancreas of a mammal to an adsorption-elution treatment using an anion-exchange resin, wherein said aqueous phase of the kallidino- genase-containing aqueous extract is brought into contact with a gel-type weakly basic anion-exchange resin to cause the kallidino- genase fraction to be adsorbed to said resin, and the kallidinogenase is eluted with an aqueous solution of an ammonium salt of a weak acid, thereby to collect the kallidinogenase. - 1 -

241130

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