Methods for generating and screening fusion protein...

C - Chemistry – Metallurgy – 40 – B

Patent

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C40B 40/02 (2006.01) C07K 16/00 (2006.01) C07K 19/00 (2006.01) C12N 15/09 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) C12P 21/02 (2006.01) C40B 30/04 (2006.01) C40B 40/10 (2006.01) C40B 50/06 (2006.01) G01N 33/53 (2006.01) G01N 33/554 (2006.01)

Patent

CA 2633138

The invention provides methods for generating fusion protein libraries, such as immuntoxin libraries. The invention also relates to libraries of recombinant cells encoding nucleic acid sequences comprising fusion proteins. In addition, the invention relates to the libraries themselves and the use of the libraries to screen for fusion proteins that are specific for target cells, such as cancer cells. Further, the invention relates to methods of improving fusion proteins and to the improved fusion proteins.

L'invention concerne des méthodes pour générer des bibliothèques de protéines hybrides, notamment des bibliothèques de cellules recombinantes codant des séquences d'acide nucléique comprenant des protéines hybrides. En outre, l'invention concerne les bibliothèques susmentionnées et leur utilisation pour cribler des protéines hybrides spécifiques à des cellules cibles, notamment des cellules cancéreuses. En outre, l'invention concerne des méthodes d'amélioration de protéines hybrides et les protéines hybrides améliorées obtenues par cette méthode.

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