Methods for generating and screening novel metabolic pathways

C - Chemistry – Metallurgy – 12 – N

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C12N 15/66 (2006.01) C12N 15/10 (2006.01) C12N 15/12 (2006.01) C12N 15/31 (2006.01) C12N 15/70 (2006.01) C12N 15/85 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2362989

The present invention relates to a novel drug discovery system for generating molecular diversity. The system provides methods for subjecting the genetic materials from a plurality of species of organisms to homologous or homeologous recombination to create novel genes and metabolic pathways. The recombined genetic materials are cloned to form recombined combinatorial gene expression libraries. Methods for screening such gene expression libraries containing recombined genes and metabolic pathways for novel activities and compounds are also provided.

L'invention concerne un système de recherche de médicaments destiné à la production d'une diversité moléculaire. Le système propose des procédés consistant à soumettre des matériels génétiques appartenant à diverses espèces d'organismes à une recombinaison homologue ou homéologue pour créer de nouveaux gènes et de nouvelles voies métaboliques. Les matériels génétiques recombinés sont clonés de façon à former des bibliothèques combinatoires recombinées d'expression de gènes. L'invention porte également sur des procédés servant à analyser lesdites bibliothèques d'expression de gènes contenant des gènes recombinés et des voies métaboliques pour détecter des activités nouvelles et des composés nouveaux.

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