Methods for generating and screening novel metabolic pathways

C - Chemistry – Metallurgy – 40 – B

Patent

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Details

C40B 40/06 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12N 15/64 (2006.01) C12N 15/66 (2006.01) C12P 1/00 (2006.01) C12P 21/02 (2006.01) C12Q 1/68 (2006.01) C40B 10/00 (2006.01) C40B 20/00 (2006.01) C40B 30/00 (2006.01) C40B 50/06 (2006.01)

Patent

CA 2219136

The present invention relates to a novel drug discovery system for generating and screening molecular diversity. The system provides methods for mixing and cloning genetic materials from a plurality of species of organisms in combinatorial gene expression libraries to generate novel metabolic pathways and classes of compounds. The system also involves methods for pre-screening or identifying for host organisms containing a library that are capable of generating such novel pathways and compounds. The host organisms may be useful in drug screening for particular diseases, and in commercial production of compounds of interest. The methods of the invention are also useful in preserving the genomes of organisms that are known or prospective sources of drugs.

La présente invention concerne un nouveau système de découverte de médicaments, destiné à générer et à cribler la diversité moléculaire. Ce système donne des procédés de mélange et de clonage de matières génétiques provenant de nombreuses espèces d'organismes, dans des bibliothèques d'expression génétique combinatoire, pour produire de nouvelles voies métaboliques et de nouvelles classes de composés. Le système concerne aussi des procédés de précriblage ou d'identification pour les organismes hôtes contenant une bibliothèque et qui sont capables de générer des voies nouvelles et des composés nouveaux de ce genre. Les organismes hôtes peuvent être utiles dans le criblage de médicaments pour des maladies particulières et dans la production commerciale de composés présentant de l'intérêt. Les procédés de l'invention sont utiles aussi dans la préservation des génomes d'organismes qui sont des sources de médicaments connues ou potentielles.

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