Methods for generating antibiotic resistant microbes and...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/02 (2006.01) C12N 1/00 (2006.01) C12N 1/20 (2006.01) C12N 15/00 (2006.01) C12N 15/10 (2006.01) C12N 15/74 (2006.01) C12Q 1/20 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2455686

The invention provides methods for generating antibiotic resistant bacteria comprising blocking mismatch repair in a bacterium to make hypermutable bacteria, contacting the bacteria with at least one antibiotic, selecting bacteria that are resistant to the antibiotic, and culturing the antibiotic resistant bacteria. The invention also provides methods of determining the genes responsible for antibiotic resistance.

L'invention concerne des procédés de production de bactéries résistant aux antibiotiques, qui comporte les étapes consistant à : bloquer dans une bactérie la réparation de mésappariements afin d'obtenir des bactéries hypermutables ; mettre en contact les bactéries avec au moins un antibiotique ; sélectionner les bactéries résistant à l'antibiotique ; et mettre en culture les bactéries résistant à l'antibiotique. L'invention concerne aussi des procédés visant à déterminer les gènes responsables de la résistance aux antibiotiques.

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