Methods for generating libraries of therapeutic...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/70 (2006.01) A61K 35/76 (2006.01) C12N 7/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2444819

The present invention is concerned with genetically labeled bacteriophages, and with methods for preparing and using the same. Genetically labeled phages may easily be distinguished from non-labeled phages. The present invention also relates to methods for selecting therapeutic bacteriophages lysing pathogenic bacteria without cross-reacting with non-pathogenic bacteria. This method permits the selection of phages which are highly specific to given pathogenic bacteria. The present invention is also concerned with methods for evaluating bacteriophage susceptibility to external genetic modifications in order to provide bacteriophages that are safe and highly specific for use in the prevention, treatment and/or control of bacterial infections or contamination in plants, animals, and humans, as well as environmental cleanup and sanitation.

La présente invention concerne des bactériophages génétiquement marqués et des procédés de préparation et d'utilisation de ces derniers. Les phages génétiquement marqués peuvent facilement être distingués des phages non marqués. L'invention concerne également des procédés permettant de sélectionner des bactériophages thérapeutiques qui lysent les bactéries pathogènes sans provoquer de réaction croisée avec les bactéries non pathogènes. Le procédé de l'invention permet de sélectionner des phages présentant une haute spécificité pour des bactéries pathogènes données. L'invention se rapporte également à des procédés permettant d'évaluer la susceptibilité des bactériophages aux modifications génétiques externes afin d'obtenir des bactériophages inoffensifs et hautement spécifiques qui seront utilisés dans la prévention, le traitement et/ou la maîtrise des infections bactériennes ou de la contamination chez les plantes, les animaux et les humains, ainsi que dans la décontamination et l'assainissement de l'environnement.

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