Methods for genetic diversification in gene conversion...

Details Methods for genetic diversification in gene conversion... Methods for genetic diversification in gene conversion...

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) C12N 5/078 (2010.01) A01K 67/027 (2006.01) C12N 15/00 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2556997

The invention relates to a modified lymphoid cell having gene conversion fully or partially replaced by hypermutation, wherein said cell has no deleterious mutations in genes encoding paralogues and analogues of the RAD51 protein, and wherein said cell is capable of directed and selective genetic diversification of a target nucleic acid by hypermutation or a combination of hypermutation and gene conversion. The invention also relates to a method for diversifying any transgenic target gene in said cell. Preferably, the target gene is integrated into the immunoglobulin light or heavy chain locus by targeted integration.

Une cellule lymphoïde modifiée ayant une conversion génétique complètement ou partiellement remplacée par hypermutation, cette cellule n'ayant pas de mutations délétères sur les gènes codant des paralogues et analogues de la protéine RAD51 et étant apte à diversifier génétiquement par orientation et sélection un acide nucléique cible au moyen de l'hypermutation ou de la combinaison de l'hypermutation et de la conversion génétique. Un procédé de diversification d'un gène cible transgénique quelconque dans cette cellule. Le gène cible est, de préférence, intégré dans un site actif à chaîne légère ou lourde d'immunoglobuline par intégration ciblée.

Affiliated with

Also associated with

Rating

Methods for genetic diversification in gene conversion... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for genetic diversification in gene conversion..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for genetic diversification in gene conversion... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1386064